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Objective To analyze the clinical characteristics in children with digestive tract poison-ing in emergency department and discuss the measures of prevention and treatment. Methods Four hundred and seventy-three cases with digestive tract poisoning who visited the emergency department of Zhengzhou Children′s Hospital were retrospectively analyzed from January 2015 to December 2017. The ages,toxic poi-soning causes,types,clinical features, laboratory examinations, hospitalization expenses and outcomes were analyzed. They were divided into drug poisoning and non-drug poisoning group to compare. Results There were 317 (67. 0%) cases aged 6 days to 3 years old,133 (28. 1%) cases aged 3 to 6 years old,23 cases (4. 9%) >6 years old. The incidence rate was similar in each season but slightly lower in winter. There were 462 (97. 7%) cases of accidental poisoning,of which 377 (79. 7%) cases were mistreated and 85 (18. 0%) cases were mistakenly fed by parents,other 11 (2. 3%) cases were non-accidental poisoning. Three hundreds and thirty-six (71. 0%) cases were drug poisoning,and 137 (29. 0%) cases were non-drug poisoning. Drug poisonings were higher in urban children than in rural children, the difference was statistically significant (χ2 ＝7. 037,P＝0. 008). The percentage of digestive symptoms and blood system symptoms in non-drug poi-soning group were higher than those in drug poisoning group,and the differences were statistically significant (54. 0% vs. 8. 3%,χ2 ＝120. 067,P<0. 001;7. 3% vs. 3. 0%,χ2 ＝4. 491,P ＝0. 034). The percentage of cardiovascular system symptoms and respiratory symptoms in the non-drug poisoning group were lower than that in the drug poisoning group,and the differences were statistically significant (1. 5% vs. 14. 9%, χ2 ＝17. 915,P<0. 001;2. 9% vs. 11. 0%,χ2 ＝8. 050,P＝0. 005). Except for liver function and myocardial en-zyme,the percentage of abnormal laboratory indicators(white blood cells,platelets,blood glucose,lactic acid, electrolyte,coagulation) in non-drug poisoning group were higher than those in the drug poisoning group,and the differences were all statistically significant(all P<0. 05). The hospitalization cost of the non-drug poison-ing group was greater than that of the drug poisoning group,and the difference was statistically significant (Z＝ －12. 444,P<0. 001). Both in the drug-poisoning group and non-drug poisoning group,the cure or im-provement rate of the <6 h treating group were higher than that of the >6h treating group,and the difference was all statistically significant(all P<0. 05). Conclusion Children with acute gastrointestinal poisoning are mostly infants and preschoolers,mainly accidental poisoning,and often taken by mistake. Drug poisonings are mostly found in cities and non-drug poisonings in rural areas. Non-drug poisoning children have more serious damages and higher hospitalization costs than drug poisoning children. Early treatment after poisoning is an important factor to improve cure rate.
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Objective@#To explore the relationship between BRAFV600E, Ki67 protein and thyroid carcinoma with different pathologic characteristics, thus to provide clinical evidence on early prognosis and personalized treatment in patients with thyroid carcinoma.@*Methods@#From January 2015 to January 2017, 76 patients diagnosed as papillary thyroid carcinoma(PTC), who treated in Yinzhou People's Hospital, were enrolled.Twenty cases with normal tumor-adjacent tissue after operation and 34 patients with non-PTC were also enrolled as control.All the patients enrolled were not treated with endocrine, chemical and radiotherapy.The expression of BRAFV600E and Ki67 protein was detected by immunohistochemical method, and the BRAFV600E and Ki67 expression and their correlation with different pathologic characteristic of thyroid carcinoma was analyzed by SPSS 18.0.@*Results@#The positive rate of BRAFV600E and Ki67 protein in 110 patients with thyroid carcinoma was 56.36%(62 cases) and 39.09%(43 cases), with the highest expression of BRAFV600E and the lowest expression of Ki67 in patients with PTC respectively.The expression of BRAFV600E and Ki67 protein was all negative in normal tumor-adjacent tissue.The positive expression of BRAFV600E and Ki67 in patients with PTC demonstrated significant difference in TNM stage, tumor side and lymphoid node metastasis(BRAFV600E: χ2=5.281, 9.771, 9.771, all P<0.05; Ki67: χ2 =7.098, 4.070, 5.067, all P<0.05), while with no obvious difference in sex, age and numbers of tumor(BRAFV600E: χ2=0.078, 0.093, 0.061, all P>0.05; Ki67: χ2=0.224, 0.518, 1.281, all P>0.05).@*Conclusion@#The detection of BRAFV600E and Ki67 is benefit on judging of innocent and malignant, the malignant degree of thyroid tissue.The high expression of BRAFV600E and Ki67 is benefit on differentiating PTC and early evaluation of prognosis.
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AIM To research the therapeutic effects of Hanbi Formula (Astragali Radix,Aconiti Radix cocta,Scorpio,Scolopendrap and Pheretima) on adjuvant-induced arthritis rats (RA) and its mechanism of action.METHODS RA rat models were established by using Freund's adjuvant,and then the rats were divided into six groups,namely control group,model group,dexamethasone positive group,Baoguang Fengshi Liquid (Notopterygii Rhizoma et Radix,Radix angelicae pubescentis,Chuanxiong Rhizoma,etc.) positive group,and low,high doses of Hanbi Formula groups.The volume and swelling of toes were measured.The interleukin-1 β (IL-1β) and tumor necrosis factor-α (TNF-α) of serum were detected by ELISA;the proliferative capacity of lymphocytes was tested by methyl thiazolyl tetrazolium (MTI) method;synovial tissue was histopathologically examined with HE staining.Finally,the expressions of interleukin-17 (IL-17) and TNF-α in synovial tissue were determined by immunohistochemical assays.RESULTS Hanbi Formula could significantly relieve toe swelling of RA rats.Compared with the model group,Hanbi Formula could significantly alleviate synovitis in rats with RA,down-regulate the expressins of IL-1 β and TNF-α in serum and synovial tissue,and inhibit lymphocyte proliferation.There were no significant differences in above indices between low-dose and high-dose Hanbi Formula groups,which was quite with Baoguang Fengshi Liquid,but less than dexamethasone.CONCLUSION Hanbi Formula possesses an obvious function of anti-RA,and its mechanism may be related to the inhibition of lymphocyte proliferation and reducing secretion of inflammatory cytokines.
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Objective To explore the mental health and personality traits of the parents of cerebral palsy children. Methods 128 parents of cerebral palsy children and other 128 parents of healthy children were investigated with the Symptom Check List-90 (SCL-90), Eysenck Personality Questionnaire (EPQ). Results The scores of SCL-90 were more in the parents of cerebral palsy children, especially in the factors of somatization, obsession, depression, anxiety, and paranoid (P<0.01). The personality traits characterized as introversion (P<0.05) and neu-roticism (P<0.01). The multiple regression showed that some factors of SCL-90 were related with the personality of neuroticism. Conclu-sion The mental health of the parents of children with cerebral palsy are poor, which may be related with the personality of neuroticism.
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Background and purpose:Papillary renal cell carcinomas (PRCC) is relatively infrequent, and there are few related researches in China. This study aimed to summarize the clinical and pathological features of PRCC, and evaluate prognostic factors for patients treated with surgery.Methods:A total of 64 patients who under-went surgery for PRCC were retrospectively assessed. PRCC tissue slides from each patient were reviewed for type (ⅠorⅡ), grade, TNM stage, coagulative tumor necrosis and microvascular invasion. We estimated overall survival using the Kaplan-Meier method. Multivariate analysis was done according to the Cox proportional hazards model of factors statistically signiifcant on univariate analysis. Results:The incidence rate of ENE was 6.04%in RCC, the median age was 55 (range 22 to 78) years. The comparison of the 22 (534.4%) typeⅠPRCCs and 42 (65.6%) typeⅡPRCCs revealed that typeⅡtumors were associated with a greater stage and grade more often. The median follow-up was 46 months (range 19 to 133). Of the 64 patients, 14 died, (4.5%) with typeⅠand 13 (31.0%) with typeⅡtumors (P=0.018). The overall survival rate was 85.7%in typeⅠtumors and 55.8%in typeⅡtumors, respectively. Univariate analysis identiifed symptoms at presentation, tumor type, TNM stage and grade as prognostic factors. On multivariate analysis only metastatic RCC remained associated with decreased overall survival (HR:14.78, P=0.004). Conclusion:The per-centage of PRCC is lower and typeⅡPRCC is relatively common compared with foreign data. Metastasis at diagnosis is an independent predictive parameter of overall survival in Chinese patients with PRCC.
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@#ObjectiveTo observe effects of combination of wax therapy and occupational therapy on spastic cerebral palsy with thumb adduction.Methods100 cases of children with spastic cerebral palsy were randomly divided into observation group(n=50) and control group(n=50). The observation group received occupational therapy and wax therapy, while the control group received only occupational therapy. The effect was compared between the two groups 3 months after treatment.ResultsThe two groups improved significantly 3 months after treatment, while the observation group was better than the control group(P＜0.001).ConclusionWax therapy combined with occupational therapy have better effects on thumb adduction in spastic cerebral palsy than simple occupational therapy.
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@#ObjectiveTo observe effects of combination of wax therapy and occupational therapy on spastic cerebral palsy with thumb adduction.Methods100 cases of children with spastic cerebral palsy were randomly divided into observation group(n=50) and control group(n=50). The observation group received occupational therapy and wax therapy, while the control group received only occupational therapy. The effect was compared between the two groups 3 months after treatment.ResultsThe two groups improved significantly 3 months after treatment, while the observation group was better than the control group(P＜0.001).ConclusionWax therapy combined with occupational therapy have better effects on thumb adduction in spastic cerebral palsy than simple occupational therapy.
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@#Objective To study the effect of brain function biofeedback on children with spastic cerebral palsy following attention deficit disorder. Methods 44 children with spastic cerebral palsy were divided into 2 groups: 23 cases in observation group and 21in control group. The routine rehabilitation including physical therapy, occupational therapy, sensory integration, speech therapy and traditional Chinese medicine were used in two groups, while at the same time EEG biofeedback therapy was used in the observation group 5 times a week for 3 months. Chinese Binet Intelligence Scale (CBIS) and The Test of Variables of Attention (TOVA) were used to assess the effect before and after treatment. Results After the treatment, the intellectual level of the observation group significantly improved (P<0.01), omissions significantly reduced (P<0.01), error number was lower (P<0.05), reaction time was significantly shorter(P<0.01), mutation rate was significantly lower (P<0.01); while the intellectual level of the control group didn't significantly improve (P>0.05), omissions and reaction time significantly reduced (P<0.01), there was not obvious difference in error number and the mutation (P>0.05). Conclusion Brain function biofeedback can effectively improve attention disorder in children with spastic cerebral palsy.
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@#ObjectiveTo observe the effects of wrist hand orthoses on cerebral palsy children with adduction of thumb. MethodsChildren with spastic hemiplegia were divided into experimental group （14 cases) and control group （15 cases）. Children in the both groups received routine rehabilitation for 3 months. Children in experimental group wore wrist hand orthoses 4～8 h/d in addition. ResultsThe passive range of motion (PROM) and scores of Fine Motor Function Measure Scale (FMFM) significantly increased in both groups after treatment. The PROM and FMFM scores in experimental groups increased more than those in the control group after treatment （P＜0.05).ConclusionWrist hand orthoses can improve the passive adduction angle of carpometacarpal joints of thumb and the fine motor function in spastic hemiplegia children after cerebral palsy.
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AIM To investigate the antithrobosis effects of glycol mannate sulfate(GMS). METHODS Vein thrombus was formed in rat by ligating postcava. Thrombus formation in vitro was observed by Chandlers method. Clotting indexes were measure by ACL200 congulation system. RESULTS GMS dosage of 20、40 mg*kg-1 could inhibit the vein thrombus formation in rats (P<0.01). GMS could inhibit the thrombosis in rabbit, in vivo, and the inhibitory action was enhanced along with the increase of dosage. The CT,TT,CT,APTT,RT and PT were prolonged, and the fibrinogen and the activity of Ⅱ and Ⅱa were decreased. The activity of ATⅢ was enhenced. CONCLUSION GMS has antithrombotic effect and the mechanism may be related to the anticongulat effect.
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The effects of eicosapentaenoic acid (EPA) on experimental thrombosis and its mechanism were studied. The results showed that eicosapentaenoic acid had a significant antithrombolic effect both in vivo and in vitro . EPA also had a fibrinolytic activity and can shorten euglobulin lysis time. Our studies also showed that EPA can inhibit platelet aggregation in rats. The plasma concentration of 6-keto-PGF1a and TXB2 was measured by radioimmunoassay. The results showed that EPA can reduce the contents of TXB2 and raise 6-keto-PGF1a/TXB2 ratio.
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The influences of propylene glycol mannate sulfate (PGMS) on experimental thrombosis and thrombolysis in vivo were studies after iv 8. 125, 6. 25, 12. 5 , 25mg/Kg in rabbits, and the effect of antithrombosis of PGMS was compared with that of heparin. The results showed that PGMS possessed remarkable effect of antithrombosis. In order to explore the mechanism of antithrombosis of PGMS, we studied the influences on the fibrinolytic and coagulant function of rabbits. The results showed that PGMS can pronouncedly prolong the prothrombin time (PT), thrombin time (TT), kaolin partial thromboplastin time (KPTT), and enhance the activity of antithrombin-III (AT -III). PGMS can cause a remarkable increase in fibrin degradation product (FDP) , shorten euglobulin lysis time (ELT) , and a decrease in the contents of fibrinogen and plasminogen activity. These results suggested that PGMS probably exert the antithrombotic effect by inhibiting coagulation and activating fibrinolysis.
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Objective To investigate the effects of glycol mannate sulfate(GMS) on thrombogenesis.Methods The thrombogenesis was observed by carotid artery and cervical vein bypass and the thrombus was weighted.Thrombogenesis in common carotid artery was induced by electric stimulation.Mice mesentery microthrombus formation was induced by laser.Pulmonary thromboembolism was induced by injection of arachidonic acid.Plasma fibrinogen of rats was measured.Results GMS 25,50 mg?kg~(-1) could decrease the weight of thrombus cohered on suture silk significantly;25,12.5 mg?kg~(-1) of GMS could prolong the obstruction time;GMS 35.7,71.4 mg?kg~(-1) could delay the formation of mice mesentery microthrombus;GMS 40,80 mg?kg~(-1) could decrease the mortality induced by arachidonic acid injection.At the dosages of 25,50 and 100 mg?kg~(-1)GMS could decrease the fibrinogen in plasma.Conclusion GMS could inhibit the thrombogenesis significantly.
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Objective: To study the protective effect of ginkgolide on rat's renal failure induced by adenine.Methods: The chronic renal functional failure model of rat was established with adenine. Its urea nitrogen (BUN), reatinine (Crea), Cholesterin (CH), total protein (TP), and albumin (ALP) in blood were determined. The pathologic changes were also observed. Results: Ginkgolide has effects on renal failure caused by adenine. Low and high dose groups decrease BUN. Crea, low dose group decrese CH, high dose group increase numbers of kindney glomerulus.Conclusion: Ginkgolide has protective effect on rat's renal failure induced by adenine.
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Objective:To study the effects of WAK on immune function in mice.Methods: After different doses of WAK were given to mice ig, its effects on delayed type hypersensitivity (DTH), hemolysin production, NK and LAK cell killing activity were observed.Results: WAK could enhance DTH and hemolysin production in immunosuppressive mice and improve NK and LAK cell killing activity in mice.Conclusions: WAK could exhibit its antitumor effect by increasing immune function.
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AIM: To study the protective effects of Naokangning (Rhizoma Chuanxiong, Radix salviae miltiorrhizae, Hirudo, etc.) against cerebral ischemia. METHODS: We observed the effect of Naokangning on mice's resistance to cerebral ischemia when bilateral common carotid arteries and vagus nerves were ligated and hypoxia under normal pressure and airtight circumstance; With the model of partial cerebral ischemia by blocking rats'middle cer ebral artery (MACO):the effects of Naokangning on the activity of superoxide dismutase (SOD) and creatine kinas e(CK), the content of malondialdehyde (MDA) and nitric oxide (NO) were measured. RESULTS: Naokangning significantly raised mice's ability of anti-cerebral ischemia and prolonged span of life in hypoxia, Moreover, it also obviously improved the activity of SOD, reduced content of MDA in cerebrum, content of NO and activity of CK in blood serum after ischemia. CONCLUSION: Naokangning could strikingly protect brain caused by cerebral ischemia.
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Experimental research showed that Gan Tang Zhi can influence blood clotting and plasma fibrinolytic system of rabbits, 15min after introvenous injecting 6.25mg/kg or 25mg/kg Gan Tang Zhi into rabbit it can pronouncedly prolong prothrombin time ( PT ) , thrombin time ( TT ) , and kaolin partial thromboplastin time ( KPTT ) ; and cause remarkable increase of fibrin degradation product ( FDP ) , Gan Tang Zhi can also shorten englobulin lysis time (ELT) and decrease fibri-nogen.
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Guan Xin Shu ( GXS ) is a heparinoid.Quails were randomly divided into 2 groups. All of them were fed with inducer diet containing 1% cholesterol and 20% fat for 6 weeks. 1 group was treated with GXS (200mg/kg/d, ip ) , the other with 0.9% NaCl(0.2ml/100g/d, ip ) . 4 and 6 weeks after administration, serum cholesterol level was significantly reduced (mean 3l%, P
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AIM To observe the effects of melatonin on acute and chronic injuries induced by oxygen and glucose deprivation (OGD) in co-cultured neuron and glia and to explore the probable mechanisms of melatonin in antagonizing the injuries. METHODS The injury model of cultured neuron and co-cultured neuron and glia was made by administration of sodium dithionite and glucose-deprived Earles solution. In neuron and glia co-culture, two different models, acute injury model at the phase of OGD and chronic injury model after 'reperfusion' were established. The levels of nitric oxide (NO) and the activity of lactate dehydrogenase (LDH) were measured by Griess reagent and LDH kits respectively. The content of malondialdehyde (MDA) was determined by TBA method. Cell viability was analyzed using colorimetric MTT assay. RESULTS Melatonin increased the level of NO at the concentration of 10 -6 , 10 -7 mol?L -1 and decreased the level of MDA content elevated by OGD at the concentration of 10 -6 , 10 -7 , 10 -8 mol?L -1 in vitro cultured cortical neurons. In the chronic injury model after 'reperfusion' melatonin (10 -6 , 10 -7 , 10 -8 mol?L -1 ) significantly decreased LDH activity and increased MTT value in neurons and glia co-cultured. But in the acute injury model, melatonin obviously increased LDH activity and decreased MTT value. CONCLUSION Melatonin protection for neuron from injuries induced by oxygen and glucose deprivation may be related to increase in the level of NO and decrease in the content of MDA. Melatonin can antagonize the injury in the chronic injury model after 'reperfusion', but exaggerate the injury in the acute injury model. These may be all related to its antioxidant action. Our results also suggest that melatonin may probably inhibit activation of microglia.